General Introduction and Aims List of Species and Strains in the IEGM Collection Ordering and Payment Information Research Activities Scientific Collaboration Staff and Publications Links  

Identification and Characterisation of Microorganisms

Identification and characterisation services offered at the IEGM Collection include classical, chemotaxonomic and genetic methods for determining the taxonomic status and phylogenetic position of microorganisms.

Only organisms up to, and including, risk group 2 (see Council Directive 93/88/EEC on the protection of workers from risks related to exposure to biological agents at work//Official Journal of European Communities, No L 268/71 of 29.10.1993) are acceptable for study.

All relevant modern taxonomic methods are in use which include:

  • Morphology
Light microscopy
Atomic force microscopy
Confocal laser scanning microscopy
  • Physiology
Physiological tests using classical test systems.
Evaluation of the data using numerical methods
  • Chemotaxonomy
Determination of the cell wall structure (amino acid and sugar composition) by TLC
Analysis of the cellular polar lipid composition by TLC
Analysis of the cellular fatty acid composition by GC-MS
Bacterial whole-cell protein electrophoresis
Immunochemical analysis
  • Molecular biological studies
Specific amplification of 16S - RNA genes using PCR
Sequencing of 16S rRNA genes
Whole genome sequencing

Other services

Identification per strain/specimen $ 180 US. Universities and educational institutes $ 125 US.

Preservation of 10 ampoules freeze-dried materials $ 110 US.

Deposit and Maintenance of Cultures

  • Fee for deposit $ 250 US   
  • Annual maintenance $ 80 US.

Training courses in the methods of isolation, cultivation and identification of alkanotrophic microorganisms $ 1200 US per 14 days.

Consultancy in biodestruction (e.g. gaseous and liquid n-alkanes, aromatics, crude oil, pharmaceuticals) and biosynthesis (e.g. fatty acids, biosurfactants) properties of alkanotrophs - on request.

Contract research - on request.

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