Identification
and Characterisation of Microorganisms
Identification and characterisation services
offered at the IEGM Collection include
classical, chemotaxonomic and genetic methods for determining the
taxonomic status and
phylogenetic position of microorganisms.
Only organisms up to, and including, risk group 2
(see Council Directive 93/88/EEC
on the protection of workers from risks related to exposure to
biological agents at
work//Official Journal of European Communities, No L 268/71 of
29.10.1993) are acceptable
for study.
All relevant modern taxonomic methods are in use
which include:
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Light microscopy
Atomic force microscopy
Confocal laser scanning microscopy |
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Physiological tests
using classical test systems.
Evaluation of the data using numerical methods |
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Determination of the
cell wall structure (amino acid and sugar composition) by TLC
Analysis of the cellular polar lipid composition by TLC
Analysis of the cellular fatty acid composition by GC-MS
Bacterial whole-cell protein electrophoresis
Immunochemical analysis |
- Molecular biological studies
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Specific amplification
of 16S - RNA genes using PCR
Sequencing of 16S rRNA genes
Whole genome sequencing
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Other
services
Identification per strain/specimen $ 180 US.
Universities and educational
institutes $ 125 US.
Preservation of 10 ampoules freeze-dried materials
$ 110 US.
Deposit and Maintenance of Cultures
- Fee for deposit $ 250
US
- Annual maintenance $ 80 US.
Training courses in the methods of isolation,
cultivation and identification of alkanotrophic microorganisms $ 1200
US
per 14 days.
Consultancy in biodestruction (e.g. gaseous and
liquid n-alkanes, aromatics, crude oil,
pharmaceuticals) and
biosynthesis (e.g. fatty acids, biosurfactants) properties of
alkanotrophs - on request.
Contract research - on request.
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